A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine max

@article{Kandoth2012AVG,
  title={A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine max},
  author={Pramod Kaitheri Kandoth and Robert Heinz and Gregory Yeckel and Nathan Wilson Gross and Parijat S Juvale and John H. Hill and Steven A. Whitham and THOMAS J. Baum and Melissa Goellner Mitchum},
  journal={BMC Research Notes},
  year={2012},
  volume={6},
  pages={255 - 255}
}
BackgroundBean pod mottle virus (BPMV) based virus-induced gene silencing (VIGS) vectors have been developed and used in soybean for the functional analysis of genes involved in disease resistance to foliar pathogens. However, BPMV-VIGS protocols for studying genes involved in disease resistance or symbiotic associations with root microbes have not been developed.FindingsHere we describe a BPMV-VIGS protocol suitable for reverse genetic studies in soybean roots. We use this method for analyzing… 
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The transcriptomes and parasitomes of various nematodes are described indicating that the effector proteins are crucial for the compatible plant nematode interactions, and various sequencing techniques used in plant-nematode genomics and transcriptomics are discussed.
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References

SHOWING 1-10 OF 25 REFERENCES
A soybean cyst nematode resistance gene points to a new mechanism of plant resistance to pathogens
TLDR
The map-based cloning of a gene at the Rhg4 (for resistance to Heterodera glycines 4) locus is reported, a major quantitative trait locus contributing to resistance to this pathogen, revealing an unprecedented plant resistance mechanism against a pathogen.
Functional analysis of the Asian soybean rust resistance pathway mediated by Rpp2.
TLDR
The results provide new insight into the signaling and biochemical pathways required for resistance against P. pachyrhizi and identify 11 genes that compromised Rpp2-mediated resistance when silenced.
Development and use of an efficient DNA-based viral gene silencing vector for soybean.
TLDR
The construction of a Cauliflower mosaic virus 35S promoter-driven BPMV vector that is efficient for the study of soybean gene function is reported, which demonstrates the utility of this new vector as an efficient tool for a wide range of applications for soybean functional genomics.
The Development of an Efficient Multipurpose Bean Pod Mottle Virus Viral Vector Set for Foreign Gene Expression and RNA Silencing1[C]
TLDR
The simultaneous expression of green fluorescent protein and silencing of phytoene desaturase shows that marker gene-assisted silencing is feasible and demonstrates the utility of this BPMV vector set for a wide range of applications in soybean and common bean, and they have implications for improvement of other plant virus-based vector systems.
Temporal and spatial Bean pod mottle virus-induced gene silencing in soybean.
TLDR
Investigation of the temporal and spatial silencing patterns achieved by Bean pod mottle virus (BPMV)-based VIGS in soybean using virus constructs targeting green fluorescence protein (GFP) discovered evidence for variable GFP silencing based on insert orientation and targeted region in the coding sequence.
Virus-induced gene silencing in tomato.
TLDR
It is shown that recombinant TRV infects tomato plants and induces efficient gene silencing, and a modified TRV vector based on the GATEWAY recombination system is constructed, allowing restriction- and ligation-free cloning of tomato ESTs.
Agrobacterium rhizogenes-mediated transformation of soybean to study root biology
TLDR
Almost 100% of the infected plants form hairy roots within 1 month from the start of the experiments, and the main roots are removed and the transgenic roots can be tested.
Virus-induced gene silencing in plants.
Applications and advantages of virus-induced gene silencing for gene function studies in plants.
TLDR
Virus-induced gene silencing is a recently developed gene transcript suppression technique for characterizing the function of plant genes that is rapid, does not require development of stable transformants, allows characterization of phenotypes that might be lethal in stable lines, and offers the potential to silence either individual or multiple members of a gene family.
Virus-induced gene silencing in tomato fruit.
TLDR
The results of this study indicate that the application of VIGS techniques by the described methods can be successfully applied to tomato fruit and is a valuable tool for studying functions of the relevant genes during fruit developing.
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