On the mechanism and some properties of vinylacetyl-CoA delta-isomerase of Clostridium kluyveri.
- ChemistryHoppe-Seyler's Zeitschrift fur physiologische Chemie
Vinylacetyl-CoA delta-isomerase from Clostridium kluyveri grown on ethanol/acetate was purified 32-fold and the extent of intramolecularity is in agreement with results of experiments conducted in 2H2O with whole cells.
The stereochemistry of vinylacetyl‐CoA‐isomerase of Clostridium kluyveri
- Chemistry, BiologyFEBS letters
Succinate-ethanol fermentation in Clostridium kluyveri: purification and characterisation of 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA Δ3-Δ2-isomerase
- Biology, ChemistryArchives of Microbiology
The finding of this dehydratase means that all of the enzymes necessary for fermentation of succinate plus ethanol by C. kluyveri have now been demonstrated to exist in this organism and confirms the proposed pathway involving a reduction of succinated via 4-hydroxybutyrate to butyrate.
Studies on the substrate range of Clostridium kluyveri; the use of propanol and succinate
- BiologyArchives of Microbiology
A mechanism of succinate metabolism to acetate was proposed which accounts for growth yield, energetics considerations, carbon balances, production of side products and intermediates.
Purification and characterization of a coenzyme-A-dependent succinate-semialdehyde dehydrogenase from Clostridium kluyveri.
- BiologyEuropean journal of biochemistry
Cell extracts of Clostridium kluyveri, grown on ethanol plus succinate contained a succinyl-CoA:CoA transferase, a coenzyme-A-dependent succinate-semialdehyde dehydrogenase, and a NAD(+)-dependent 4-hydroxybutyrate dehydrogenases, which suggest a ping-pong mechanism.
Utilization of (E)-2-butenoate (Crotonate) by Clostridium kluyveri and some other Clostridium species
- BiologyArchives of Microbiology
Crotonate seems not to be a very special carbon source since C. butyricum and C. pasteurianum grow on crotonate medium supplemented by peptone and yeast extract.
Dehydrogenases involved in the conversion of succinate to 4-hydroxybutanoate by Clostridium kluyveri
- BiologyApplied and environmental microbiology
Data support the hypothesis that C. kluyveri uses succinate as an electron acceptor for the reducing equivalents generated from the ATP-producing oxidation of ethanol.
The enzymic conversion of 3cis- and 3ans-alkenoyl-CoA esters into their 2trans-isomers.
- Chemistry, BiologyBiochimica et biophysica acta
Molecular analysis of the anaerobic succinate degradation pathway in Clostridium kluyveri
- Biology, EngineeringJournal of bacteriology
A region of genomic DNA from Clostridium kluyveri was cloned in Escherichia coli by a screening strategy which was based on heterologous expression of the clostridial 4-hydroxybutyrate dehydrogenase gene, and similarities to the adhE (aad) gene products from E. coli were revealed.
Metabolic properties of Eubacterium pyruvativorans, a ruminal 'hyper-ammonia-producing' anaerobe with metabolic properties analogous to those of Clostridium kluyveri.
- Biology, MedicineMicrobiology
The fermentative niche of E. pyruvativorans appears to be to scavenge amino acids, lactate and possibly other metabolites in order to generate ATP via acetate formation, using volatile fatty acid elongation with C(2) units derived from other substrates to dispose of reducing equivalents.
SHOWING 1-10 OF 14 REFERENCES
Coenzym A: Eine einfache Synthese von S-Acylderivaten des Pantetheins
Es wird eine einfache Methode zur Herstellung von (+)-S-Acetylpantethein (Ia) und (+)-S-Benzoyl-pantethein (Ib) beschrieben. Durch Ammonolyse des kristallisierten Ib lasst sich leicht freies…
Darstellung und Wirkungen von Acetyl‐Derivaten des Cysteamins
Athylenimin reagiert mit Thioessigsaure unter Bildung von N-Acetyl-cysteamin, das durch Keten in N.S-Diacetyl-cysteamin und durch Essigsaureanhydrid in N.N.S-Triacetyl-cysteamin verwandelt werden…
Enzymes of fatty acid metabolism. I. General introduction; crystalline crotonase.
- Biology, MedicineThe Journal of biological chemistry
On the evaluation of the constants Vm and KM in enzyme reactions.
The Mechanism of the Decarboxylation of α,β- and β,γ-Unsaturated Malonic Acid Derivatives and the Course of Decarboxylative Condensation Reactions in Pyridine1