A versiconal hemiacetal acetate converting enzyme in aflatoxin biosynthesis

@article{Hsieh2004AVH,
  title={A versiconal hemiacetal acetate converting enzyme in aflatoxin biosynthesis},
  author={Dennis P. H. Hsieh and C. C. Wan and Julia Billington},
  journal={Mycopathologia},
  year={2004},
  volume={107},
  pages={121-126}
}
Conversion of the aflatoxin biosynthetic intermediate versiconal hemiacetal acetate (VHA) in a cell free extract ofAspergillus parasiticus ATCC 15517 is investigated. The enzymatic reaction is monitored by a method using high performance liquid chromatography (HPLC). The major product of the enzymatic reaction is a water soluble compound not chloroform-extractable at pH 7.5. The product becomes chloroform extractable upon acidification of the reaction medium and is separated and quantitated by… Expand
Timing of appearance of versiconal hemiacetal acetate esterase and versiconal cyclase activity in cultures ofAspergillus parasiticus
The sequence of steps versiconal hemiacetal acetate (VHA) to versiconal (VL) catalyzed by an esterase and VL to versicolorin B (VB) catalyzed by VL cyclase has been previously demonstrated inExpand
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