A versatile toolbox for PCR‐based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes

@article{Janke2004AVT,
  title={A versatile toolbox for PCR‐based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes},
  author={C. Janke and M. Magiera and N. Rathfelder and C. Taxis and Simone Reber and H. Maekawa and A. Moreno-Borchart and G. Doenges and E. Schwob and E. Schiebel and M. Knop},
  journal={Yeast},
  year={2004},
  volume={21}
}
Tagging of genes by chromosomal integration of PCR amplified cassettes is a widely used and fast method to label proteins in vivo in the yeast Saccharomyces cerevisiae. [...] Key Result The tags include the so far brightest and most yeast-optimized version of the red fluorescent protein, called RedStar2, as well as all other commonly used fluorescent proteins and tags used for the detection and purification of proteins and protein complexes. Using the provided cassettes for N- and C-terminal gene tagging or for…Expand
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