A versatile system for functional analysis of genes and microRNAs in cotton.

@article{Gu2014AVS,
  title={A versatile system for functional analysis of genes and microRNAs in cotton.},
  author={Zhouhang Gu and Changjun Huang and Fangfang Li and Xueping Zhou},
  journal={Plant biotechnology journal},
  year={2014},
  volume={12 5},
  pages={638-49}
}
Cotton is an important economic crop worldwide. Due to its long growth cycle, large genome size and recalcitrance to stable transformation, traditional methods for the analysis of gene function in this crop are difficult and labour intensive. Here, we report a cotton leaf crumple virus (CLCrV)-based vector and its application in gene function analysis through virus-induced gene silencing (VIGS) and overexpression of microRNAs (miRNAs), small tandem target mimic (STTM) and artificial miRNA… CONTINUE READING
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