A versatile strategy for rapid conditional genome engineering using loxP sites in a small synthetic intron in Plasmodium falciparum

@inproceedings{Jones2016AVS,
  title={A versatile strategy for rapid conditional genome engineering using loxP sites in a small synthetic intron in Plasmodium falciparum},
  author={Matthew L. Jones and Sujaan Das and Hugo Belda and Christine R Collins and Michael J. Blackman and Moritz Treeck},
  booktitle={Scientific reports},
  year={2016}
}
Conditional genome engineering in the human malaria pathogen Plasmodium falciparum remains highly challenging. Here we describe a strategy for facile and rapid functional analysis of genes using an approach based on the Cre/lox system and tailored for organisms with short and few introns. Our method allows the conditional, site-specific removal of genomic sequences of essential and non-essential genes by placing loxP sites into a short synthetic intron to produce a module (loxPint) can be… CONTINUE READING
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Conditional expression of apical membrane antigen 1 in Plasmodium falciparum shows it is required for erythrocyte invasion by merozoites

  • A Yap
  • Cell Microbiol 16,
  • 2014
1 Excerpt

Adaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytes

  • Moon, W R.
  • Proc Natl Acad Sci USA 110,
  • 2013

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