A versatile shuttle cosmid vector for use in Escherichia coli and actinomycetes.

@article{Denis1992AVS,
  title={A versatile shuttle cosmid vector for use in Escherichia coli and actinomycetes.},
  author={François Denis and Ryszard Brzezinski},
  journal={Gene},
  year={1992},
  volume={111 1},
  pages={115-8}
}
A shuttle cosmid vector has been constructed for Escherichia coli and actinomycetes. This vector, pFD666, utilizes the origin of replication (ori) of the broad-host-range plasmid, pJV1, from Streptomyces phaeochromogenes, for replication in actinomycetes and is compatible with vectors derived from pIJ101. The pFD666 vector employs the neomycin phosphotransferase-encoding gene (neo) from transposon Tn5 as the selective marker. To achieve this, the native promoter of neo was replaced by one… CONTINUE READING