A versatile plasmid biosensor useful to identify quorum sensing LuxR-family orphans in bacterial strains.

@article{Steindler2008AVP,
  title={A versatile plasmid biosensor useful to identify quorum sensing LuxR-family orphans in bacterial strains.},
  author={Laura Steindler and Giulia Devescovi and Sujatha Subramoni and Vittorio 7 Venturi},
  journal={Journal of microbiological methods},
  year={2008},
  volume={73 3},
  pages={273-5}
}
Eight luxI-family gene promoters (luxI, cviI, ahlI, rhlI, cepI, phzI, traI and ppuI) were cloned in tandem, upstream a promoterless lacZ gene in a promoter probe vector yielding pMULTIAHLPROM. This unique construct is useful in determining whether a bacterial strain not producing N-acyl homoserine lactone signal molecules (AHLs) possesses orphan LuxR type proteins able to respond to AHLs and activate transcription from quorum sensing target genes. Using pMULTIAHLPROM, it was demonstrated that… CONTINUE READING

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Experiments in Molecular Genetics

J. H. Miller
Cold Spring Harbor Laboratory, • 1972
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Highly Influenced

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