A versatile microtiter assay for the universal cdc2 cell cycle regulator.

@article{Ducommun1990AVM,
  title={A versatile microtiter assay for the universal cdc2 cell cycle regulator.},
  author={Bernard Ducommun and David S. Beach},
  journal={Analytical biochemistry},
  year={1990},
  volume={187 1},
  pages={94-7}
}
A microassay for p34cdc2 based on the high affinity association between cdc2 and Schizosaccharomyces pombe p13suc1 has been developed. p13 purified from Escherichia coli was immobilized on microtiter plates and cellular lysate was incubated in the wells to allow the binding of cdc2 and its associated proteins. p34cdc2 was assayed either as a histone kinase… CONTINUE READING