A versatile method of identifying speciWc binding proteins on aYnity resins

Abstract

The isolation of both speciWc and nonspeciWc binding proteins on aYnity matrices bearing bioactive compounds hinders the identiWcation of drug cellular targets. Although solid-phase elution and competition methods conventionally are used to distinguish between speciWc and nonspeciWc receptor–ligand interactions, these approaches often are severely restricted by low ligand solubility and/or slow kinetic dissociation. This article describes an alternative and versatile method, termed serial aYnity chromatography, to identify ligand receptors using aYnity resins. © 2006 Elsevier Inc. All rights reserved.

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Cite this paper

@inproceedings{Yamamoto2006AVM, title={A versatile method of identifying speciWc binding proteins on aYnity resins}, author={Kiyoshi Yamamoto and Akira Yamazaki and Mikio Takeuchi and Akito Tanaka}, year={2006} }