A versatile assay for gelatinases using succinylated gelatin.

  title={A versatile assay for gelatinases using succinylated gelatin.},
  author={Vijaykumar M Baragi and Benjamin J. Shaw and Richard R Renkiewicz and Paul J. Kuipers and Howard G. Welgus and Mahesh Mathrubutham and Jon R. Cohen and Srinath Rao},
  journal={Matrix biology : journal of the International Society for Matrix Biology},
  volume={19 3},
A spectrophotometric assay using succinylated gelatin as substrate is described for measuring the catalytic activity of gelatinases. The assay is based on measurement of primary amines exposed as a result of hydrolysis of the substrate by gelatinases. Comparison of hydrolysis by matrix metalloproteinase (MMP) 1, 2, 3, 7, 9 indicated that succinylated gelatin was primarily digested by MMP-2 and -9. The assay is rapid (<60 min), specific, suitable for measuring gelatinolytic activity of enzymes… CONTINUE READING

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