A versatile and scalable two-step ion-exchange chromatography process for the purification of recombinant adeno-associated virus serotypes-2 and -5.

@article{Brument2002AVA,
  title={A versatile and scalable two-step ion-exchange chromatography process for the purification of recombinant adeno-associated virus serotypes-2 and -5.},
  author={Nicole Brument and Robert Morenweiser and V{\'e}ronique Blouin and Estelle Toublanc and Isabelle Raimbaud and Yan Ch{\'e}rel and S{\'e}bastien Folliot and Florence Gaden and Pierre A. Boulanger and Gabrielle Kroner-Lux and Philippe Moullier and Fabienne Rolling and Anna Salvetti},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2002},
  volume={6 5},
  pages={678-86}
}
Here we describe the development of a two-step chromatography process based on the use of ion-exchange resins for the purification of recombinant adeno-associated virus (rAAV) serotypes-2 and-5. In vitro and in vivo results demonstrate that this method, which does not require any prepurification step of the cell lysate, can be applied to obtain highly pure rAAV2 and rAAV5 stocks. As such,this procedure can be easily transferred in vector cores and also scaled up, allowing the direct comparison… CONTINUE READING

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