A vector-free microfluidic platform for intracellular delivery.

@article{Sharei2013AVM,
  title={A vector-free microfluidic platform for intracellular delivery.},
  author={Armon Sharei and Janet Zoldan and Andrea Adamo and Woo Young Sim and Nahyun Kate Cho and Emily G. Jackson and Shirley Mao and Sabine Schneider and Min-Joon Han and Abigail K R Lytton-Jean and Pamela A. Basto and Siddharth Jhunjhunwala and Jungmin Lee and Daniel A. Heller and Jeon Woong Kang and George C Hartoularos and Kwang-Soo Kim and Daniel G Anderson and Robert Langer and Klavs F Jensen},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2013},
  volume={110 6},
  pages={
          2082-7
        }
}
Intracellular delivery of macromolecules is a challenge in research and therapeutic applications. Existing vector-based and physical methods have limitations, including their reliance on exogenous materials or electrical fields, which can lead to toxicity or off-target effects. We describe a microfluidic approach to delivery in which cells are mechanically deformed as they pass through a constriction 30-80% smaller than the cell diameter. The resulting controlled application of compression and… CONTINUE READING

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References

Publications referenced by this paper.
SHOWING 1-10 OF 44 REFERENCES

Endosomal escape pathways for delivery of biologicals.

  • Journal of controlled release : official journal of the Controlled Release Society
  • 2011
VIEW 1 EXCERPT

A novel intracellular protein delivery platform based on singleprotein nanocapsules

M Yan
  • Nat Nanotechnol
  • 2010
VIEW 2 EXCERPTS