A vacuolar-type proton pump energizes K+/H+ antiport in an animal plasma membrane.

@article{Wieczorek1991AVP,
  title={A vacuolar-type proton pump energizes K+/H+ antiport in an animal plasma membrane.},
  author={Helmut Wieczorek and M. Putzenlechner and Wolfgang Zeiske and Uwe K. A. Klein},
  journal={The Journal of biological chemistry},
  year={1991},
  volume={266 23},
  pages={15340-7}
}
In this paper we demonstrate that a vacuolar-type H(+)-ATPase energizes secondary active transport in an insect plasma membrane and thus we provide an alternative to the classical concept of plasma membrane energization in animal cells by the Na+/K(+)-ATPase. We investigated ATP-dependent and -independent vesicle acidification, monitored with fluorescent acridine orange, in a highly purified K(+)-transporting goblet cell apical membrane preparation of tobacco hornworm (Manduca sexta) midgut… CONTINUE READING
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