A vaccinia virus transfer vector using a GUS reporter gene inserted into the I4L locus.

@article{Howley1996AVV,
  title={A vaccinia virus transfer vector using a GUS reporter gene inserted into the I4L locus.},
  author={Paul M. Howley and Dani{\`e}le Spehner and Robert Drillien},
  journal={Gene},
  year={1996},
  volume={172 2},
  pages={233-7}
}
A vaccinia virus (VV) transfer vector is described which enables integration of heterologous sequences into the I4L locus (ribonucleotide reductase-encoding gene) through co-insertion of a GUS selection marker. I4L- VV recombinants formed blue plaques when an agarose overlay containing XGluc (5-bromo-4-chloro-3-indolyl-beta-glucuronide) was added to the infected cell monolayer. Viruses already containing a lacZ reporter gene were also suitable recipients for the selection procedure since… CONTINUE READING
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