A user's guide to restriction enzyme-mediated integration in Dictyostelium

@article{Guerin2002AUG,
  title={A user's guide to restriction enzyme-mediated integration in Dictyostelium},
  author={Nicholas A. Guerin and Denis A. Larochelle},
  journal={Journal of Muscle Research & Cell Motility},
  year={2002},
  volume={23},
  pages={597-604}
}
Restriction enzyme-mediated integration (REMI) has been used to study a number of cellular and developmental processes in Dictyostelium discoideum. In this paper we review the basics of this powerful method of introducing random mutations in Dictyostelium. Here we discuss several mutation screens that have been devised and some of the genes that have been discovered through this approach to mutagenesis. Included in this discussion is how one goes about isolating a gene that has been disrupted… CONTINUE READING

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