A universal method for directional cloning of PCR products based on asymmetric PCR.

Abstract

We have developed a novel protocol for directional cloning of PCR products into any vector. The target sequence is amplified in two parallel asymmetric PCRs using specially but simply designed primers. Two single-stranded products are produced and they are annealed to form a double-stranded DNA fragment bearing overhangs at both ends that correspond to the… (More)
DOI: 10.1042/BA20070210

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