A universal RNAi-based logic evaluator that operates in mammalian cells

  title={A universal RNAi-based logic evaluator that operates in mammalian cells},
  author={Keller Rinaudo and Leonidas Bleris and Rohan Maddamsetti and Sairam Subramanian and Ron Weiss and Yaakov Benenson},
  journal={Nature Biotechnology},
Molecular automata that combine sensing, computation and actuation enable programmable manipulation of biological systems. We use RNA interference (RNAi) in human kidney cells to construct a molecular computing core that implements general Boolean logic to make decisions based on endogenous molecular inputs. The state of an endogenous input is encoded by the presence or absence of 'mediator' small interfering RNAs (siRNAs). The encoding rules, combined with a specific arrangement of the siRNA… 

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