A ubiquitous chromatin opening element prevents transgene silencing in pluripotent stem cells and their differentiated progeny.

@article{Pfaff2013AUC,
  title={A ubiquitous chromatin opening element prevents transgene silencing in pluripotent stem cells and their differentiated progeny.},
  author={Nils Pfaff and Nico Lachmann and Mathias Ackermann and Saskia Kohlscheen and Christian Brendel and Tobias Maetzig and Heiner Niemann and Michael N Antoniou and Manuel Grez and Michellle Cross and Tobias Cantz and Thomas Moritz},
  journal={Stem cells},
  year={2013},
  volume={31 3},
  pages={
          488-99
        }
}
Methylation-induced gene silencing represents a major obstacle to efficient transgene expression in pluripotent cells and thereof derived tissues. As ubiquitous chromatin opening elements (UCOE) have been shown to prevent transgene silencing in cell lines and primary hematopoietic cells, we hypothesized a similar activity in pluripotent cells. This concept was investigated in the context of cytidine deaminase (CDD) gene transfer, an approach to render hematopoietic cells resistant to the… CONTINUE READING
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