A two-step approach for systematic identification and quality evaluation of wild and introduced Anemone flaccida Fr. Schmidt (Di Wu) based on DNA barcode and UPLC-QTOF-MS/MS

@article{Hu2020ATA,
  title={A two-step approach for systematic identification and quality evaluation of wild and introduced Anemone flaccida Fr. Schmidt (Di Wu) based on DNA barcode and UPLC-QTOF-MS/MS},
  author={Xin Hu and Man Liu and Yifei Liu and Jingjing Zhang and Yuwen Tang and Hong Pei and Hegang Liu and Shilin Chen and Chengwu Song and Zhigang Hu},
  journal={Analytical and Bioanalytical Chemistry},
  year={2020},
  volume={412},
  pages={1807 - 1816}
}
Herbal materials have both medicinal and commercial values. As such, accurate species and content identification and verification are necessary to ensure the safe and effective use for medical and commodity purposes. Herein, we introduce a two-step approach for systematic identification and quality evaluation of wild and introduced Anemone flaccida Fr. Schmidt (aka Di Wu) using DNA barcode and ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS… Expand
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References

SHOWING 1-10 OF 50 REFERENCES
Investigation on Species Authenticity for Herbal Products of Celastrus Orbiculatus and Tripterygum Wilfordii from Markets Using ITS2 Barcoding
TLDR
The efficacy of the ITS2 barcode for the traceability of C. orbiculatus and T. wilfordii is supported, and the present study provides one method and reference for the identification of the herbal materials and adulterants in the medicinal markets. Expand
Validation of the ITS2 Region as a Novel DNA Barcode for Identifying Medicinal Plant Species
TLDR
The second internal transcribed spacer (ITS2) of nuclear ribosomal DNA represents the most suitable region for DNA barcoding applications and can be potentially used as a standard DNA barcode to identify medicinal plants and their closely related species. Expand
Characterization and identification of steroidal saponins from the seeds of Trigonella foenum-graecum by ultra high-performance liquid chromatography and hybrid time-of-flight mass spectrometry.
  • L. Kang, Yang Zhao, +7 authors B. Ma
  • Chemistry, Medicine
  • Journal of pharmaceutical and biomedical analysis
  • 2013
TLDR
Low-performance liquid chromatography coupled with hybrid quadrupole time-of-flight mass spectrometry was used to rapidly separate and identify steroidal saponins from the crude extract of the seeds of Trigonella foenum-graecum (TFG). Expand
[The species traceability of the ultrafine powder and the cell wall-broken powder of herbal medicine based on DNA barcoding].
TLDR
Using ITS2 barcode can accurately and effectively distinguish ultrafine powder and cell wall-broken powder of herbal medicine in the quality control and market supervision. Expand
Identification and characterization of curcuminoids in turmeric using ultra-high performance liquid chromatography-quadrupole time of flight tandem mass spectrometry.
TLDR
The fragmentation behaviors of the 15 types of curcuminoids were clearly illustrated in this work, which will be helpful for detection and identification of corresponding trace cur cuminoids in complex turmeric samples using UHPLC-QTOF-MS/MS methods. Expand
A renaissance in herbal medicine identification: from morphology to DNA.
TLDR
This review summarizes recent key advances in the DNA barcoding of medicinal plant ingredients (herbal materia medica) as a contribution towards safe and efficacious herbal medicines. Expand
Comprehensive Analysis of the Triterpenoid Saponins Biosynthetic Pathway in Anemone flaccida by Transcriptome and Proteome Profiling
TLDR
A combination of the de novo transcriptome and proteome profiling based on the Illumina HiSeq 2000 sequencing platform and iTRAQ technique was shown to be a powerful method for the discovery of candidate genes, which encoded enzymes that were responsible for the biosynthesis of novel secondary metabolites in a non-model plant. Expand
RAPD Profile for Authentication of Medicinal Plant Glycyrrhiza glabra Linn.
TLDR
The obtained unique amplicons in PCR amplification clearly distinguished genuine as well as adulterant samples having similar morphology and thus, RAPD helping to serve as a complementary tool for quality control. Expand
Quality control of the traditional Chinese medicine Ruyi jinhuang powder based on high-throughput sequencing and real-time PCR
TLDR
An effective approach to screen the biological and toxical composition of TCM based on high-throughput sequencing (HTS), as well as fast detection and validation of the toxical species by real-time PCR, based on ITS2 DNA barcoding is established. Expand
[Study on high throughput sequencing identification of Fructus Arctii and five counterfeit species mix power].
  • Y. Xing, Si-you Chen, +5 authors T. Kang
  • Biology, Medicine
  • Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
  • 2018
TLDR
Using ITS2 sequences as DNA barcodes, high-throughput sequencing technology can be used to detect the Fructus Arctii and its spurious specie in mixed powder, which can provide reference for the quality control, safe use of medicinal materials and the identification of mixed powder of traditional Chinese medicine. Expand
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