A two-step antibody strategy for surface plasmon resonance spectroscopy detection of protein-DNA interactions in nuclear extracts.

@article{Su2008ATA,
  title={A two-step antibody strategy for surface plasmon resonance spectroscopy detection of protein-DNA interactions in nuclear extracts.},
  author={X. Su and Siew Jun Neo and Wendy Y X Peh and J. Thomsen},
  journal={Analytical biochemistry},
  year={2008},
  volume={376 1},
  pages={
          137-43
        }
}
  • X. Su, Siew Jun Neo, +1 author J. Thomsen
  • Published 2008
  • Biology, Medicine
  • Analytical biochemistry
  • Surface plasmon resonance (SPR) spectroscopy has emerged as a powerful alternative to conventional biochemistry methods for studying protein-DNA interactions that involve recombinant proteins of known identity. There are, however, limited demonstrations of SPR detection of protein-DNA bindings in crude samples, e.g., cell extracts, where the challenge is to detect and identify specific DNA binding protein(s) among other protein components in a physiological setting. We have developed a two-step… CONTINUE READING
    19 Citations
    Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance
    • 53
    • PDF
    Detection of Protein-DNA Binding in Crude Nuclear Extract Using Biacore Assay
    Synthetic Oligonucleotide Probes for Detection of Autoimmune Antibodies
    • 1
    • PDF
    Sensitive detection of unlabeled oligonucleotides using a paired surface plasma waves biosensor.
    • 4

    References

    SHOWING 1-10 OF 25 REFERENCES
    Label-free immunosensing for alpha-fetoprotein in human plasma using surface plasmon resonance.
    • 12
    Steroidogenic factor 1-DNA binding: a kinetic analysis using surface plasmon resonance.
    • 7
    • PDF