• DOI:10.1016/j.ab.2008.02.002
Corpus ID: 30570767

A two-step antibody strategy for surface plasmon resonance spectroscopy detection of protein-DNA interactions in nuclear extracts.

@article{Su2008ATA,
  title={A two-step antibody strategy for surface plasmon resonance spectroscopy detection of protein-DNA interactions in nuclear extracts.},
  author={X. Su and Siew Jun Neo and Wendy Y X Peh and J. Thomsen},
  journal={Analytical biochemistry},
  year={2008},
  volume={376 1},
  pages={
          137-43
        }
}

X. Su, Siew Jun Neo, +1 author J. Thomsen

Published 2008

Biology, Medicine

Analytical biochemistry

Surface plasmon resonance (SPR) spectroscopy has emerged as a powerful alternative to conventional biochemistry methods for studying protein-DNA interactions that involve recombinant proteins of known identity. There are, however, limited demonstrations of SPR detection of protein-DNA bindings in crude samples, e.g., cell extracts, where the challenge is to detect and identify specific DNA binding protein(s) among other protein components in a physiological setting. We have developed a two-step… CONTINUE READING

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