A two sodium ion/D-glucose symport mechanism: membrane potential effects on phlorizin binding.

@article{Lever1984ATS,
  title={A two sodium ion/D-glucose symport mechanism: membrane potential effects on phlorizin binding.},
  author={Julia E. Lever},
  journal={Biochemistry},
  year={1984},
  volume={23 20},
  pages={4697-702}
}
Apical membrane vesicles isolated from a continuous renal cell line, LLC-PK1, catalyze electrogenic Na+-stimulated hexose transport and Na+-dependent binding of 3H-labeled 1-[2-(beta-D-glucopyranosyloxy)-4, 6-dihydroxyphenyl]-3-(4-hydroxyphenyl)-1-propanone [( 3H]phlorizin), a competitive ligand of this transport system. Phlorizin was not itself transported across the membrane and thus can serve as a probe of the binding step. The stoichiometry of Na+-dependent phlorizin binding in vesicles was… CONTINUE READING