A two-site monoclonal antibody ELISA for the quantification of the major Dermatophagoides spp. allergens, Der p I and Der f I.

@article{Luczynska1989ATM,
  title={A two-site monoclonal antibody ELISA for the quantification of the major Dermatophagoides spp. allergens, Der p I and Der f I.},
  author={Christina M Luczynska and Luisa Karla de Paula Arruda and Thomas A Platts-Mills and J David Miller and M. Lopez and Martin D. Chapman},
  journal={Journal of immunological methods},
  year={1989},
  volume={118 2},
  pages={227-35}
}
A two-site monoclonal antibody (Mab) ELISA was developed to measure the Group I allergens from Dermatophagoides spp., Der p I from D. pteronyssinus and Der f I from D. farinae. Species-specific Mabs were used to coat microtiter plates which were then incubated with allergen or house dust extracts. Bound allergen was detected using a biotinylated Mab which recognized a common epitope on both Der p I and Der f I, followed by the addition of streptavidin-peroxidase and ABTS/H2O2 substrate. The… CONTINUE READING
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Identification and quantitation of cockroach allergen using monoclonal antibodies

  • S. M. Pollart, T.A.E. Platts-Mills, M. D. Chapman
  • (Mab) New Engl Reg. Allergy Proc
  • 1988

Identification and quantitation of cockroach allergen using monoclonal antibodies ( Mab ) New Engl Reg

  • S. M. Pollart, T. A. E. Platts-Mills, M. D. Chapman
  • Allergy Proc .
  • 1988

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