A two-plasmid system for stable, selective-pressure-independent expression of multiple extracellular proteins in mycobacteria.

@article{Harth2004ATS,
  title={A two-plasmid system for stable, selective-pressure-independent expression of multiple extracellular proteins in mycobacteria.},
  author={Guenter Harth and Sa{\vs}a Masle{\vs}a-Gali{\'c} and Marcus A. Horwitz},
  journal={Microbiology},
  year={2004},
  volume={150 Pt 7},
  pages={
          2143-51
        }
}
Recombinant mycobacteria expressing Mycobacterium tuberculosis extracellular proteins are leading candidates for new vaccines against tuberculosis and other mycobacterial diseases, and important tools both in antimycobacterial drug development and basic research in mycobacterial pathogenesis. Recombinant mycobacteria that stably overexpress and secrete major extracellular proteins of M. tuberculosis in native form on plasmids pSMT3 and pNBV1 were previously constructed by the authors. To… CONTINUE READING

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