A two-photon and second-harmonic microscope.

@article{Nikolenko2003ATA,
  title={A two-photon and second-harmonic microscope.},
  author={Volodymyr Nikolenko and Boaz A Nemet and Rafael Yuste},
  journal={Methods},
  year={2003},
  volume={30 1},
  pages={3-15}
}
Two-photon microscopy has revolutionized life sciences by enabling long-term imaging of living preparations in highly scattering tissue while minimizing photodamage. At the same time, commercial two-photon microscopes are expensive and this has prevented the widespread application of this technique to the biological community. As an alternative to commercial systems, we provide an update of our efforts designing custom-built two-photon instruments by modifying the Olympus FluoView laser… CONTINUE READING

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