A two-insult in vitro model of PMN-mediated pulmonary endothelial damage: requirements for adherence and chemokine release.

@article{Wyman2002ATI,
  title={A two-insult in vitro model of PMN-mediated pulmonary endothelial damage: requirements for adherence and chemokine release.},
  author={Travis H Wyman and A Jason Bjornsen and David J. Elzi and Conrad W. Smith and Kelly M England and Marguerite R. Kelher and Christopher C. Silliman},
  journal={American journal of physiology. Cell physiology},
  year={2002},
  volume={283 6},
  pages={C1592-603}
}
Lysophosphatidylcholines (lyso-PCs), generated during blood storage, are etiologic in a two-insult, sepsis-based model of transfusion-related acute lung injury (TRALI). Individually, endotoxin (LPS) and lyso-PCs prime but do not activate neutrophils (PMNs). We hypothesized that priming of PMNs alters their reactivity such that a second priming agent causes PMN activation and endothelial cell damage. PMNs were primed or not with LPS and then treated with lyso-PCs, and oxidase activation and… CONTINUE READING