A two gene – two promoter system for enhanced expression of a restorer gene (barstar) and development of improved fertility restorer lines for hybrid seed production in crop plants

@article{Bisht2004ATG,
  title={A two gene – two promoter system for enhanced expression of a restorer gene (barstar) and development of improved fertility restorer lines for hybrid seed production in crop plants},
  author={Naveen C Bisht and Arun Jagannath and Vibha Gupta and Pradeep Kumar Burma and Deepak Pental},
  journal={Molecular Breeding},
  year={2004},
  volume={14},
  pages={129-144}
}
We report in this study, strategies for enhancing and extending tissue-specific expression of a restorer gene (barstar) and their use in the development of improved fertility restorer lines for transgenic male sterile (barnase) lines for hybrid seed production. In the first strategy, a chimeric promoter was developed by combining regulatory elements of two overlapping tapetum-specific promoters (A9 and TA29) by placing a 275bp fragment of the A9 promoter (minus the TATA box) upstream to a… CONTINUE READING