A two-dimensional electrophoresis map of Chinese hamster ovary cell proteins based on fluorescence staining.

Abstract

We report on the development of a detailed two-dimensional electrophoresis map of Chinese hamster ovary (CHO) cell proteins based on fluorescence staining and tandem time-of-flight (TOF/TOF)-mass spectrometry. We observed a 71% success rate in the identification of proteins even though the CHO genome is not sequenced. The map consists of 224 protein identifications present in 274 two-dimensional gel electrophoresis (2-DE) gel spots. We have also initiated a study of the phosphoproteome using a commercially available phosphoprotein-specific fluorescent stain. Using this stain, we observe 672 phosphorylated proteins, including many proteins known to be phosphorylated, which is 36% of the proteins we visualized with a total protein stain and consistent with expectations.

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@article{Hayduk2004ATE, title={A two-dimensional electrophoresis map of Chinese hamster ovary cell proteins based on fluorescence staining.}, author={Eric J Hayduk and Leila H. Choe and Kelvin H. Lee}, journal={Electrophoresis}, year={2004}, volume={25 15}, pages={2545-56} }