A tunable refractive index matching medium for live imaging cells, tissues and model organisms

  title={A tunable refractive index matching medium for live imaging cells, tissues and model organisms},
  author={Tobias Boothe and Lennart Hilbert and Michael Heide and Lea Berninger and Wieland B. Huttner and Vasily Zaburdaev and Nadine L. Vastenhouw and Eugene Wimberly Myers and David N. Drechsel and Jochen Christian Rink},
In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus substantially improves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral… 

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