A trypsin-sensitive receptor on membrane vesicles is required for nuclear envelope formation in vitro

@article{Wilson1988ATR,
  title={A trypsin-sensitive receptor on membrane vesicles is required for nuclear envelope formation in vitro},
  author={Katherine L Wilson and John F. L. Newport},
  journal={The Journal of Cell Biology},
  year={1988},
  volume={107},
  pages={57 - 68}
}
The reformation of functioning organelles at the end of mitosis presents a problem in vesicle targeting. Using extracts made from Xenopus laevis frog eggs, we have studied in vitro the vesicles that reform the nuclear envelope. In the in vitro assay, nuclear envelope growth is linear with time. Furthermore, the final surface area of the nuclear envelopes formed is directly dependent upon the amount of membrane vesicles added to the assay. Egg membrane vesicles could be fractionated into two… CONTINUE READING

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