A triple mutation in the second transmembrane domain of mouse dopamine transporter markedly decreases sensitivity to cocaine and methylphenidate.

@article{Chen2005ATM,
  title={A triple mutation in the second transmembrane domain of mouse dopamine transporter markedly decreases sensitivity to cocaine and methylphenidate.},
  author={Rong Chen and Dawn D. Han and Howard Haogang Gu},
  journal={Journal of neurochemistry},
  year={2005},
  volume={94 2},
  pages={352-9}
}
Previously, we reported that Phe105 in transmembrane domain 2 of the mouse dopamine transporter (DAT) is crucial for high-affinity cocaine binding. In the current study, we investigated whether other residues surrounding Phe105 also affect the potency of cocaine inhibition. After three rounds of sequential random mutagenesis at these residues, we found a… CONTINUE READING