A transposon-based activation-tagging population in Arabidopsis thaliana (TAMARA) and its application in the identification of dominant developmental and metabolic mutations.

@article{Schneider2005ATA,
  title={A transposon-based activation-tagging population in Arabidopsis thaliana (TAMARA) and its application in the identification of dominant developmental and metabolic mutations.},
  author={Anja Schneider and Thomas Kirch and Tamara Gigolashvili and Hans P Mock and Uwe Sonnewald and R{\"u}diger Simon and U. -I. Fl{\"u}gge and Wolfgang Werr},
  journal={FEBS letters},
  year={2005},
  volume={579 21},
  pages={4622-8}
}
A population of 9471 stable activation-tagged lines was generated by transposable element mediated activation tagging mutagenesis in Arabidopsis (TAMARA) using the maize En/Spm transposon system. Based on DNA gel blot and flanking sequence analysis, this population contains approximately 6000 independent transposon insertions. A greenhouse-based screen identified six dominant or semi-dominant activation tagged mutants with obvious developmental alterations, among these a new pistillata mutant… CONTINUE READING

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