A transgenic medaka line with visible markers for genotypic and phenotypic sex.

@article{Kanamori2013ATM,
  title={A transgenic medaka line with visible markers for genotypic and phenotypic sex.},
  author={Akira Kanamori and Keiko Toyama},
  journal={Environmental science & technology},
  year={2013},
  volume={47 12},
  pages={
          6640-5
        }
}
Accurate genotyping of sex is required for correct interpretation in any in vivo assays with endocrine disrupting chemicals (EDCs). Visible markers for genotypic sex, if reliable, simplify assays because time-consuming PCR-based genotyping can be skipped. Here, we describe a line of Japanese medaka with a brain-expressed green fluorescent protein (GFP) transgene inserted near the sex-determining locus. When used with a white pigment cell marker, genotypic sex can be determined reliably as early… CONTINUE READING
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