A transformation system for the nonuniversal CUG(Ser) codon usage species Candida rugosa.

@article{Tang2003ATS,
  title={A transformation system for the nonuniversal CUG(Ser) codon usage species Candida rugosa.},
  author={S J Tang and Kuang-Hui Sun and Guang-Huan Sun and Terng-Yuan Chang and Wan-Lin Wu and Guan-Chiun Lee},
  journal={Journal of microbiological methods},
  year={2003},
  volume={52 2},
  pages={231-8}
}
Since Candida rugosa utilizes a nonuniversal serine codon CUG rather than leucine, no vectors have been constructed to transform this organism. Moreover, it is difficult to design a new transformation system because no selection markers and promoters are available. In this study, Zeocin (400 microg/ml) was demonstrated to inhibit the growth of C. rugosa. The dominant selectable marker bleomycin-resistant determinant (ble) gene containing five CUG codons in an open-reading frame of 375 bp was… CONTINUE READING

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