A transformation method for obtaining marker-free plants based on phosphomannose isomerase

  title={A transformation method for obtaining marker-free plants based on phosphomannose isomerase},
  author={Karin Sonntag and Youping Wang and Michael Wallbraun},
A selectable marker system for plant transformation of rape plants that does not require the use of antibiotics or herbicides was developed. The selectable marker consists of the phosphomannose isomerase (PMI) gene from E. coli under the control of a plant promoter. The PMI gene was transferred to Brassica napus by Agrobacteriummediated transformation, which allows the selection of transgenic plants with mannose and sucrose as selective agents. The highest transformation frequency of 7.9 % was… CONTINUE READING
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Phosphomannose isomerase: An efficient selectable marker for plant transformation

In Vitro Cellular & Developmental Biology - Plant • 2001
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Parameters interacting with mannose selection for the production of transgenic sugar plant

M. Joersbo, S. G. Petersen, F. T. Okkels
Physiol. Plant. 105: 109–115. • 1999

GUS fusions: ß-glucuronidase as a sensitive and versatile gene fusion marker in high plants

R. A. Jefferson, T. A. Kavanagh, M. W.Bevan
EMBO J. 6: 3901–3907. • 1987