A transcription factor IIB homolog from the hyperthermophilic archaeon Pyrococcus furiosus binds Zn or Fe in an N-terminal Cys4 motif

@article{Zeng1996ATF,
  title={A transcription factor IIB homolog from the hyperthermophilic archaeon Pyrococcus furiosus binds Zn or Fe in an N-terminal Cys4 motif},
  author={Qiandong Zeng and Lara M. Lewis and Christopher M. Colangelo and Jiaowang Dong and Robert Scott},
  journal={JBIC Journal of Biological Inorganic Chemistry},
  year={1996},
  volume={1},
  pages={162-168}
}
 The gene for an archaeal homolog of the eukaryotic transcription factor TFIIB has been cloned from the marine hyperthermophilic archaeon Pyrococcus furiosus and overexpressed in Escherichia coli. This TFB gene displays a sequence that is identical to a gene sequence in P. woesei. A gene for the 49-residue N-terminal domain of TFB that contains a putative C-X2-C-X15-C-X2-C metal-binding motif was subcloned and overexpressed as TFB-NTD. Purification of the TFB-NTD gene product yields Zn- and Fe… CONTINUE READING

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