A top-down proteomics approach for differentiating thermal resistant strains of Enterobacter sakazakii.

@article{Williams2005ATP,
  title={A top-down proteomics approach for differentiating thermal resistant strains of Enterobacter sakazakii.},
  author={Tracie L. Williams and Steven R. Monday and Sharon G. Edelson-Mammel and Robert Buchanan and Steven M. Musser},
  journal={Proteomics},
  year={2005},
  volume={5 16},
  pages={4161-9}
}
Thermal tolerance has been identified as an important factor relevant to the pathogenicity of Enterobacter sakazakii in human neonates. To identify a biomarker specific for this phenotypic trait, intact protein expression profiles of 12 strains of E. sakazakii were obtained using liquid chromatography mass spectrometry. Proteins were extracted from the bacterial cells, separated by reversed-phase liquid chromatography and mass analyzed. At the end of the chromatography run, the uncharged masses… CONTINUE READING

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