A tobacco nuclear extract supporting transcription, processing, splicing and modification of plant intron-containing tRNA precursors.

@article{Yukawa2001ATN,
  title={A tobacco nuclear extract supporting transcription, processing, splicing and modification of plant intron-containing tRNA precursors.},
  author={Yasushi Yukawa and Hao Fan and Kazuhito Akama and H. Beier and H. J. Gross and Masahiro Sugiura},
  journal={The Plant journal : for cell and molecular biology},
  year={2001},
  volume={28 5},
  pages={583-94}
}
Nuclear tRNA genes are transcribed by RNA polymerase III (Pol III) and pre-tRNAs are processed into mature tRNAs via complex processes in the nucleus. We have developed an in vitro Pol III-dependent transcription system derived from tobacco cultured cells, which supports efficiently not only transcription of a variety of plant tRNA genes but also 5'-and 3… CONTINUE READING