A tobacco cryptic constitutive promoter, tCUP, revealed by T-DNA tagging

@article{Foster1999ATC,
  title={A tobacco cryptic constitutive promoter, tCUP, revealed by T-DNA tagging},
  author={Elizabeth Foster and Jiro Hattori and H{\'e}l{\`e}ne Labb{\'e} and Th{\'e}r{\`e}se Ouellet and Pierre R. Fobert and Lisa E. James and V. N. Iyer and Brian L. A. Miki},
  journal={Plant Molecular Biology},
  year={1999},
  volume={41},
  pages={45-55}
}
We have isolated a constitutive promoter sequence, tCUP, from tobacco by T-DNA tagging using a promoterless GUS-nos3′ reporter gene construct. The T-DNA integration event produced a translational fusion with the GUS gene that is expressed widely in organs, at both the mRNA and enzyme activity levels. In tobacco transformed with a tCUP-GUS-nos3′ gene, GUS specific activity in leaves was within a range of values similar to those of plants transformed with the widely used constitutive promoter… CONTINUE READING

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