A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene

@article{Gillies1983ATT,
  title={A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene},
  author={Stephen D Gillies and Sherie L. Morrison and Vernon T. Oi and Susumu Tonegawa},
  journal={Cell},
  year={1983},
  volume={33},
  pages={717-728}
}
We have studied the DNA sequences required for high level expression of a cloned heavy chain immunoglobulin gene stably introduced into mouse myeloma cells by DNA transfection. We found that DNA sequences derived from the germ line JH-C mu region are required for accurate and efficient transcription from a functionally rearranged VH promoter. Similar to viral transcriptional enhancer elements, these cellular sequences stimulate transcription from either the homologous VH gene segment promoter… 
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Direct evidence is shown for a functionally similar enhancer within the large κ gene intron of the mouse which is, however, less active than the heavy-chain gene enhancer.
The endogenous immunoglobulin heavy chain enhancer can activate tandem VH promoters separated by a large distance
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The heavy chain enhancer appears to be capable of activating transcription of a VH promoter located 17.5 kb away and of activating two tandem VH promoters.
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