A tissue-specific protein purification approach in Caenorhabditis elegans identifies novel interaction partners of DLG-1/Discs large

@inproceedings{Waaijers2016ATP,
  title={A tissue-specific protein purification approach in Caenorhabditis elegans identifies novel interaction partners of DLG-1/Discs large},
  author={Selma Waaijers and Javier Mu{\~n}oz and Christian W. H. Berends and Jo{\~a}o Jacob Ramalho and Soenita S Goerdayal and Teck Y Low and Adja D Zoumaro-Djayoon and Michael Hoffmann and Thijs Koorman and Roderick P. Tas and Martin Harterink and Stefanie Seelk and Jana Kerver and Casper C. Hoogenraad and Olaf Bossinger and Baris Tursun and Sander van den Heuvel and Albert J. R. Heck and Mike Boxem},
  booktitle={BMC Biology},
  year={2016}
}
Affinity purification followed by mass spectrometry (AP/MS) is a widely used approach to identify protein interactions and complexes. In multicellular organisms, the accurate identification of protein complexes by AP/MS is complicated by the potential heterogeneity of complexes in different tissues. Here, we present an in vivo biotinylation-based approach for the tissue-specific purification of protein complexes from Caenorhabditis elegans. Tissue-specific biotinylation is achieved by the… CONTINUE READING