A tissue culture infection test in routine rabies diagnosis.

@article{Webster1987ATC,
  title={A tissue culture infection test in routine rabies diagnosis.},
  author={Walter Archibald Webster},
  journal={Canadian journal of veterinary research = Revue canadienne de recherche veterinaire},
  year={1987},
  volume={51 3},
  pages={
          367-9
        }
}
A cell culture infection test was developed for the isolation of rabies virus from field cases submitted for rabies diagnosis. The procedure involved the addition of a suspension of suspect brain tissue to a suspension of murine neuroblastoma cells in 96-well microtiter plates. The cultures were then incubated at 35-36 degrees C for four days at which time they were fixed, stained with a fluorescein-labelled hamster antirabies antibody conjugate and examined with a fluorescence microscope… CONTINUE READING

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