A tightly regulated high level expression vector that utilizes a thermosensitive lac repressor: production of the human T cell receptor V beta 5.3 in Escherichia coli.

@article{Andrews1996ATR,
  title={A tightly regulated high level expression vector that utilizes a thermosensitive lac repressor: production of the human T cell receptor V beta 5.3 in Escherichia coli.},
  author={Brenda Andrews and Hedy Adari and Gerhard Hannig and Elaine Lahue and Myl{\`e}ne Gosselin and Simone Martin and Abdoulaziz Ahmed and Pamella J. Ford and Edward Hayman and Savvas C Makrides},
  journal={Gene},
  year={1996},
  volume={182 1-2},
  pages={101-9}
}
A series of vectors has been constructed to express the human T cell receptor V beta 5.3 under the control of the hybrid trc promoter in Escherichia coli. Transcriptional induction of the trc promoter was achieved chemically by using isopropyl beta-D-thiogalactopyranoside (IPTG) in a bacterial strain that harbors the lacIq gene, or thermally by using the mutant lacIts gene that encodes a temperature-sensitive lac repressor [Bukrinsky et al. (1988) Multicopy expression vector based on… CONTINUE READING

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