A three-phase liquid chromatographic method for delta13C analysis of amino acids from biological protein hydrolysates using liquid chromatography-isotope ratio mass spectrometry.

@article{Smith2009ATL,
  title={A three-phase liquid chromatographic method for delta13C analysis of amino acids from biological protein hydrolysates using liquid chromatography-isotope ratio mass spectrometry.},
  author={Colin I Smith and Benjamin T Fuller and Kyungcheol Choy and Michael P Richards},
  journal={Analytical biochemistry},
  year={2009},
  volume={390 2},
  pages={165-72}
}
We report a three-phase chromatographic method for the separation and analysis of delta(13)C values of underivatized amino acids from biological proteins (keratin, collagen, and casein) using liquid chromatography-isotope ratio mass spectrometry (LC-IRMS). Both precision and accuracy of delta(13)C values for standard amino acid mixtures over the range of approximately 8 to 1320 ng of carbon per amino acid on the column were assessed. The precision of delta(13)C values of amino acids was found… CONTINUE READING

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