A three-kilobase fragment of the human Robo4 promoter directs cell type-specific expression in endothelium.

@article{Okada2007ATF,
  title={A three-kilobase fragment of the human Robo4 promoter directs cell type-specific expression in endothelium.},
  author={Yoshiaki Okada and Kiichiro Yano and Enjing Jin and Nobuaki Funahashi and Mie Kitayama and Takefumi Doi and Katherine C. Spokes and David L. Beeler and S C Shih and Hitomi Okada and Tatyana A Danilov and Elizabeth Maynard and Takashi Minami and P. Oettgen and William C. Aird},
  journal={Circulation research},
  year={2007},
  volume={100 12},
  pages={1712-22}
}
Robo4, a member of the roundabout family, is expressed exclusively in endothelial cells and has been implicated in endothelial cell migration and angiogenesis. Here we report the cloning and characterization of the human Robo4 promoter. The 3-kb 5'-flanking region directs endothelial cell-specific expression in vitro. Deletion and mutation analyses revealed the functional importance of two 12-bp palindromic DNA sequences at -2528 and -2941, 2 SP1 consensus motifs at -42 and -153, and an ETS… CONTINUE READING