A three-disulphide derivative of hen lysozyme. Structure, dynamics and stability.

@article{Radford1991ATD,
  title={A three-disulphide derivative of hen lysozyme. Structure, dynamics and stability.},
  author={Sheena E Radford and Derek N. Woolfson and Stephen R. Martin and Gordon Lowe and Christopher M. Dobson},
  journal={The Biochemical journal},
  year={1991},
  volume={273(Pt 1)},
  pages={211-7}
}
A three-disulphide derivative of hen egg-white lysozyme was made by selective reduction and carboxymethylation of one of the four original disulphide bridges. N-Terminal sequencing and two-dimensional 1H-n.m.r. spectroscopy revealed that the disulphide bridge linking cysteine residues 6 and 127 had been modified and that the three remaining disulphide bonds were native-like in nature. Analysis of COSY and NOESY spectra indicated that the three-disulphide lysozyme (CM6.127-lysozyme retains the… CONTINUE READING

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