A three-dimensional culture system using alginate hydrogel prolongs hatched cattle embryo development in vitro.

@article{Zhao2015ATC,
  title={A three-dimensional culture system using alginate hydrogel prolongs hatched cattle embryo development in vitro.},
  author={Shuan Zhao and Zhen-xing Liu and Hui Gao and Yi Wu and Yuan Yi Fang and Shuai-Shuai Wu and Ming-jie Li and Jia-Hua Bai and Yan Liu and Alexander C O Evans and Shen-Ming Zeng},
  journal={Theriogenology},
  year={2015},
  volume={84 2},
  pages={184-92}
}
No successful method exists to maintain the three-dimensional architecture of hatched embryos in vitro. Alginate, a linear polysaccharide derived from brown algae, has characteristics that make it an ideal material as a three-dimensional (3D) extracellular matrix for in vitro cell, tissue, or embryo culture. In this study, alginate hydrogel was used for IVC of posthatched bovine embryos to observe their development under the 3D system. In vitro-fertilized and parthenogenetically activated… CONTINUE READING
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