A thiophilic adsorbent for the one-step high-performance liquid chromatography purification of monoclonal antibodies.

Abstract

Thiophilic adsorption chromatography, developed originally by Porath and colleagues (1985, FEBS Lett. 185, 306-310) for conventional chromatographic techniques, was transformed to the HPLC mode upon preparation of new and improved sulfur-containing silica beads. The new thiophilic adsorbent is of high capacity and is suitable for the rapid and single-step purification of all subclasses of monoclonal and polyclonal antibodies. Due to its broad specificity, the thiophilic silica column is an efficient, stable, and inexpensive substitute for protein A and protein G columns used today to purify antibodies.

Cite this paper

@article{Nopper1989ATA, title={A thiophilic adsorbent for the one-step high-performance liquid chromatography purification of monoclonal antibodies.}, author={Benjamin Nopper and Fortune Kohen and Meir Wilchek}, journal={Analytical biochemistry}, year={1989}, volume={180 1}, pages={66-71} }