A thermostable variant of fructose bisphosphate aldolase constructed by directed evolution also shows increased stability in organic solvents.

@article{Hao2004ATV,
  title={A thermostable variant of fructose bisphosphate aldolase constructed by directed evolution also shows increased stability in organic solvents.},
  author={Jijun Hao and Alan Berry},
  journal={Protein engineering, design & selection : PEDS},
  year={2004},
  volume={17 9},
  pages={689-97}
}
Thermostable variants of the Class II fructose bisphosphate aldolase have been isolated following four rounds of directed evolution using DNA shuffling of the fda genes from Escherichia coli and Edwardsiella ictaluri. Variants from all four generations of evolution have been purified and characterized. The variants show increased thermostability with no… CONTINUE READING