A thermostable, alkaline-active, keratinolytic proteinase from chrysosporium keratinophilum.

@article{Dozie1994ATA,
  title={A thermostable, alkaline-active, keratinolytic proteinase from chrysosporium keratinophilum.},
  author={Ikechukwu N S Dozie and Charles N Okeke and N C Unaeze},
  journal={World journal of microbiology & biotechnology},
  year={1994},
  volume={10 5},
  pages={563-7}
}
Thermostable alkaline proteinase was produced by a strain of Chrysosporium keratinophilum when cultured in lactose/mineral salt medium incorporating keratin solubilized with DMSO. The proteinase, partially purified by cold-acetone precipitation followed by gel-filtration on Sephadex G-75, was optimally active at pH 9 and stable from pH 7 to 10 with over 90% relative residual activity after incubation at 25°C for 24 h. The optimum temperature for enzyme activity was 90°C at which the activity… CONTINUE READING
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